General Information

  • ID:  hor004589
  • Uniprot ID:  P18758
  • Protein name:  Thymosin beta-4
  • Gene name:  tmsb4
  • Organism:  Xenopus laevis (African clawed frog)
  • Family:  Thymosin beta family
  • Source:  Animal
  • Expression:  Spleen, kidney, heart, and oocytes.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:  Xenopus (subgenus), Xenopus (genus), Xenopodinae (subfamily), Pipidae (family), Pipoidea (superfamily), Anura (order), Batrachia (superorder), Amphibia (class), Tetrapoda, Dipnotetrapodomorpha, Sarcopterygii (superclass), Euteleostomi, Teleostomi, Gnathostomata, Vertebrata, Craniata (subphylum), Chordata (phylum), Deuterostomia, Bilateria, Eumetazoa, Metazoa (kingdom), Opisthokonta, Eukaryota (superkingdom), cellular organisms
  • GO MF:  GO:0003779 actin binding; GO:0003785 actin monomer binding
  • GO BP:  GO:0007015 actin filament organization
  • GO CC:  GO:0005737 cytoplasm; GO:0005856 cytoskeleton

Sequence Information

  • Sequence:  SDKPDMAEIEKFDKAKLKKTETQEKNPLPSKETIEQEKQTSES
  • Length:  43(2-44)
  • Propeptide:  MSDKPDMAEIEKFDKAKLKKTETQEKNPLPSKETIEQEKQTSES
  • Signal peptide:  NA
  • Modification:  T1 N-acetylserine
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Plays an important role in the organization of the cytoskeleton.
  • Mechanism:  Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-P18758-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    hor004589_AF2.pdbhor004589_ESM.pdb

Physical Information

Mass: 571482 Formula: C212H352N56O78S
Absent amino acids: CGHRVWY Common amino acids: K
pI: 4.72 Basic residues: 9
Polar residues: 9 Hydrophobic residues: 7
Hydrophobicity: -170.93 Boman Index: -14910
Half-Life: 1.9 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 40.93
Instability Index: 6793.49 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  3124756
  • Title:  Thymosin beta 4Xen: a new thymosin beta 4-like peptide in oocytes of Xenopus laevis.